pLX304 Luciferase-V5 blast

LOCUS       pLX304 Luciferase-V5 blast                9375 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral vector for constitutive expression of Luciferase with 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    pLX304 Luciferase-V5 blast
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Bajikar SS, Wang CC, Borten MA, Pereira EJ, Atkins KA, Janes KA
  TITLE     Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor 
            Sequestration in Triple-Negative Breast Cancer.
  JOURNAL   Dev Cell. 2017 Nov 20;43(4):418-435.e13. doi: 
            10.1016/j.devcel.2017.10.027.
  PUBMED    29161592
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     112..131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     365..382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        535..565
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    573..589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     578..600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     597..613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     597..613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     632..652
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        634..652
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        680..906
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             907..1087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1134..1259
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1752..1985
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2170..2214
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2389..2889
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2808..2826
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2901..3296
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3356..3473
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3590..3893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3894..4097
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4048..4068
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4120..4144
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4147..5796
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4213..4231)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(5798..5822)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5824..5865
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5907..6495
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5960..5980)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6378..6389)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6567..6800
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6878..6999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6915..6934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6969..6988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7039..7174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7101..7120
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7194..7213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7195..7213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7223..7240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7223..7239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7232..7254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7381..7836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7468..7487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7678..7699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7862..7966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7967..8827
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8185..8204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8998..211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
       61 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      301 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      361 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      421 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      481 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      541 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      601 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      661 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
      721 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      781 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      841 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      901 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      961 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     1021 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     1081 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     1141 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     1201 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1261 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1321 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1381 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1441 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1501 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1561 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1621 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1681 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1741 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1801 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1861 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1921 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1981 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     2041 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     2101 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     2161 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     2221 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     2281 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     2341 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     2401 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     2461 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     2521 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     2581 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     2641 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     2701 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     2761 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     2821 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     2881 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     2941 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     3001 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     3061 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     3121 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     3181 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     3241 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     3301 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     3361 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     3421 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     3481 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat
     3541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     3601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     3661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     3721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     3781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     3841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     3901 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     3961 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     4021 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     4081 tctatataag cagagctctc tggctaactg tcgggatcaa caagtttgta caaaaaagtt
     4141 ggcaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
     4201 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
     4261 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
     4321 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
     4381 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
     4441 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
     4501 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
     4561 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
     4621 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     4681 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
     4741 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
     4801 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     4861 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     4921 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag
     4981 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
     5041 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
     5101 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
     5161 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     5221 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     5281 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
     5341 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     5401 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg
     5461 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
     5521 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt
     5581 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     5641 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     5701 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     5761 ctcataaagg ccaagaaggg cggaaagatc gccgtgttgc caactttctt gtacaaagtg
     5821 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
     5881 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5941 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6001 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6061 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6121 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6181 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6241 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6301 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6361 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6421 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6481 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     6541 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6601 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6661 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6721 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6781 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6841 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7021 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7081 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     7141 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     7201 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     7381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7801 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8701 agcactgggg ccagatggta aaccctcccg tatcgtagtt atctacacga cggggagtca
     8761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9361 caccgaactg agata
//